Energetics of Biological Macromolecules, Part B, Volume 295 (Methods in Enzymology)

General Description of the Volume:
The very existence of biological structures and their functional interactions are dictated by energetic relationships. Thus the central theme of this volume is that thermodynamic methods, i.e. techniques that probe the energetics of biological macromolecules, now comprise a powerful and practical family of tools for research in modern biology. The application of thermodynamics and statistical thermodynamics to biochemical and biophysical systems is presented. This volume supplements Methods in Enzymology, Volume 259.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Key Features
* Major topics covered include:
* Deciphering rules of helix stability in peptides
* Protein Folding in Membranes
* Molecular Crowding
* Study of the Bohr Effect in Hemoglobin Intermediates
* Photoacoustic Calorimetry of Proteins
* Theoretical Aspects of Isothermal Titration Calorimetry
* Energetic Methods to Study Bifunctional Biotin Repressor

Annotation

Audience: Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Editorial Reviews

This volume honors K.E. van Holde for his many contributions to studies of energetics of protein folding, protein assembly and biologic recognition, and ligand-ligand interaction. It includes 24 chapters by experts who cover areas including determination of the energetics of protein-bilayer interactions; molecular design of peptide ligands; study of the Bohr effect in hemoglobin interactions; and photoacoustic calorimetry of proteins and studies of interactions and folding of repressor proteins. This volume is meant to cover topics to which van Holde made contributions and to include newer methologic approaches and applications that should be of general interest. While some of these chapters are very specific, they illustrate the potential of these applications to other study systems. Nonetheless, this volume was not meant to be a general volume that focuses on only the most commonly used methods. The audience could include anyone from student to experienced structural biologist or protein chemist. Each chapter is well written, very concise, yet very informative, and should be interesting reading for investigators at different levels of training. This book should be informative to anyone with a need to understand how to characterize protein structure and activities and an interest in the specific methods and applications presented. The features include many informative illustrations with very useful, very extensive bibliographies. The book is in the form of the attractive Methods in Enzymology series. This would be a worthwhile addition to the library of anyone with interests in energetics of protein folding and protein ligand interaction. Since the editorsand contributors are internationally recognized experts, the content is very meaningful.