Applications Of Plant Biotechnology: In Vitro Propagation, Plant Transformations And Secondary Metabolite Production

This book provides a general introduction of plant tissue culture followed by specific applications of biotechnology in regeneration of rice (Oryza sativa), Maize, Eucalyptus, hot pepper, guava (Psidium guajava L.) stone fruit (Pinus pinea) and compares the features of in vitro grown plants to in vivo plants, Transgenic plants production and application, generating marker-free transgenic plants, genetic engineering and metabolic engineering of plants, molecular farming, abiotic stress tolerance, transgenic in floriculture and ornamental plants, celery, Secondary metabolite production with special reference to sennoside, genetic transformation of potato and biosafety concerns, bioinformatics and its application to crop improvement, Intellectual property rights, biotechnological aspects of secondary metabolite production, application of biotechnology in pharmaceutical sciences and production of recombinant proteins, cyclotides, Hypericum perforatum and Gentiana punctata provide a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. Besides covering basic techniques employed in leading laboratories worldwide, follows an extended account of important applications in, for example, plant propagation, gene technology and secondary metabolite production. The book will prove useful to both students and researchers of biotechnology, agriculture, horticulture, forestry as well as for the industry.

Table Of Contents:

1. Tissue Culture: Historical Perspectives and ApplicationsSection I: In Vitro Plant Regeneration2. High Frequency Plant Regeneration via Multiple Shoot Induction from Shoot Apex in Indica Rice (Oryza sativa L.) Varieties3. Somatic and Gametic Embryogenesis in Maize: Cell Biology and Applications4. Somatic Embryogenesis in Eucalyptus—An Update to 20095. Increased Embryo Production by Manipulation of Pretreatment Materials and Media in Isolated Microspore Culture of Hot Pepper (Capsicum annuum L.)6. Biotechnological Advancement in Getting In Vitro Regeneration and Mass Multiplication in Guava (Psidium guajava L.): A Success Story in Fruit Trees7. In Vitro Propagation of Stone Pine (Pinus pinea L.) Molecular and Physiological Bases8. Morphological, Anatomical and Physiological Characteristics of in vitro Grown Plants in Comparison to ex vitro Grown PlantsSection II: Transgenic Plants and their Applications9. Production of Transgenic Plants and Their Application in Biotechnology10. Practical Application of Genetic Transformation11. Generating Marker-free Transgenic Plants12. Role of recA/Rad51 Gene Family in Homologous Recombination, Repair and Genetic Engineering of Transgenic Plants13. Metabolic Engineering in Plants14. Molecular Farming for Production of Biologically Important Molecules in Plants15. Improving Crop Tolerance to Abiotic Stresses by Plant Biotechnology16. Transgenic Floricultural and Ornamental Plants17. Flux in Morphology and GUS Expression in Somatic Embryogenic-Derived Populations of Transgenic Celery18. The Potato (Solanum tuberosum L.): An Overview of Genetic Transformation and Related Biosafety Issues19. Clean DNA Transformation of Rice Using Minimal Gene-Expression-Cassettes to Minimize Biosafety Concerns20. Bioinformatics Application to Crop Improvement21. Intellectual Property Rights, Biopiracy & Patent RevocationSection III: Secondary Metabolites and medicinal plants22. Biotechnological Aspects of Secondary Metabolite Production23. Applications of Plant Biotechnology in Pharmaceutical Sciences: A Critical Review24. Production of Recombinant Proteins in Plants for Pharmaceutical Use25. Intraspecific Variation of Plant Secondary Metabolites26. Optimisation Steps Towards Bioprocess Development for the Production of Cyclotides27. Genetic (In)stability in Hypericum perforatum L.: Physiological and Induced Sources of Genetic Variation28. Biotechnology of Endangered Medicinal Plants: Spotted Gentian, Gentiana punctata L.29. Direct Shoot Regeneration of Cassia senna for Sustainable Production of Anthraquinone Glycoside-Sennoside IndexAuthor Index